Journal: Plant Biotechnology Journal

Article Title: A large‐scale whole‐exome sequencing mutant resource for functional genomics in wheat

doi: 10.1111/pbi.14111

Figure Lengend Snippet: Overview of the method used to establish the wheat mutant database by exome capture sequencing. Dry wheat seeds were treated with ethyl methanesulfonate, γ‐rays, or C‐ion beam irradiation. M 1 or M 2 plants were propagated by single seed descent; for each M 2 line, M 3 plants were grown in a row to obtain seed stocks for distribution. Finally, 1719 M 2 lines and 443 high generation lines were used for exome capture sequencing. The mutation information was deposited into a public database.

Article Snippet: We established a public database of these wheat mutations based on the mutations detected by exome capture sequencing ( http://jing411.molbreeding.com/#/ ) to broadly disseminate and promote the efficient use of these mutants by the plant research community.

Techniques: Mutagenesis, Sequencing, Irradiation

Journal: Plant Biotechnology Journal

Article Title: A large‐scale whole‐exome sequencing mutant resource for functional genomics in wheat

doi: 10.1111/pbi.14111

Figure Lengend Snippet: Gene mapping of the yellow‐green leaf gene from the wheat mutant population. (a) Phenotype of the yellow‐green leaf mutant yg1 . (b) Identifying the linked SNPs by exome capture sequencing. The SNPs that were homozygous WT alleles in green leaved plants and homozygous or heterozygous mutant alleles in yellow‐green leaved plants were identified as the candidate mutations linked to the yellow‐green leaf gene. The first three dark‐green plants to the left of the dotted line represent the green leaved individuals, while the remaining plants following the dotted line represent the yellow‐green leaved individuals. (c) Mapping of the yellow‐green leaf gene. Black lines indicate the WT genotype; white lines indicate the homozygous mutant genotype; grey lines indicate the heterozygous genotype. The number of individuals with the corresponding genotype and phenotype are shown in parentheses. Red dotted lines indicate the physical interval of the yellow‐green leaf gene, as determined by phenotype and genotype analyses.

Article Snippet: We established a public database of these wheat mutations based on the mutations detected by exome capture sequencing ( http://jing411.molbreeding.com/#/ ) to broadly disseminate and promote the efficient use of these mutants by the plant research community.

Techniques: Mutagenesis, Sequencing